FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378922
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81600.2414971402121742No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74260.21977423568818694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54090.1600806411038787No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42130.12468473672964336No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT36280.10737152263354999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG19350.042.1221771
TATACGA19750.040.9678342
ATACGAC22250.036.3647083
GTATCAA357150.035.3314321
GACACTC25150.031.453977
TACGACA25750.031.190894
TATCAAC417400.030.0461862
ATGGGAG43200.028.7828455
ATCAACG438400.028.4712243
TCAACGC438900.028.411684
CAACGCA446800.028.0824265
AACGCAG450750.027.7274556
ACGACAC29350.027.1623845
GGTATCA252500.026.0123081
TACACCG11750.025.8228115
ACGCAGA498800.024.898087
CGCAGAG499350.024.8774058
AGAGTGT53500.024.68382824-25
GAGTGTC25250.024.02616726-27
CCCTTAG7800.023.6449451
Produced by FastQC (version 0.11.5)