FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5214151
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC243520.4670367237159031No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT137720.26412737183867513No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT128370.24619540170585777No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT94300.18085398754274665No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC92860.17809227235651595No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA92450.1773059506715475No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC90050.17270309202782966No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA89010.17070851994888525No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT83720.16056305235502386No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG79760.15296833559288942No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC79530.1525272283061998No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC75200.14422290416982556No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG74450.14278451084366373No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG71130.13641722305318738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62540.11994282482421395No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC61930.11877293158560234No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT61550.11804414563368033No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA60830.116663288040565No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG59890.11486050173844219No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG56480.1083206067488264No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT53920.10341089086219407No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC53240.10210674757980734No Hit
GTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGGTTAGCGCAAG53010.1016656402931177No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG52890.10143549736093183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA258950.088.401371
GGTATCA132100.072.6201861
TATCAAC399150.057.6209872
ATCAACG432600.053.2819943
AACGCAG439450.052.7625966
CGCAGAG439500.052.4454888
ACGCAGA441550.052.2345967
TCAACGC445200.051.6770324
CAACGCA447450.051.5780035
GCAGAGT450100.050.981669
CTATTGA43800.038.2780889
CTGTGCA126500.038.1680879
TCTCTGC22650.037.490738
ACTGTGC137200.037.34538
CACTGTG130300.037.3346757
GCTATTG47200.035.981578
TTTAGGG50350.034.4451142
ACACAGC142100.033.7293321
GGGCTAT52050.032.490566
GGCTATT52600.032.150837
Produced by FastQC (version 0.11.5)