FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004918902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732520
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA85480.3128247917673064Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT82970.30363913164405015Clontech Universal Primer Mix Long (96% over 26bp)
TTCTCATTCTTCCTTCCTGTCTTGCTACCCTCACAGCCTCATCCAAAAGA39680.1452139417094843No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT38610.1412981423740723No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37490.13719936176130457Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33430.12234128204002166Clontech Universal Primer Mix Long (96% over 26bp)
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT29480.1078857611289213No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC29400.10759299108515216No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGT27480.10056651003469326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)