FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4815622
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169650.35229093977891124No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT106040.22020000739260678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93320.19378597406524017No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC90560.1880546272111889No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA86380.17937454393222724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84900.1763012130104896No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT79790.16568991503070632No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA77820.16159906238487987No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC72920.15142384514399176No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA69210.1437197520901765No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT62370.1295159794518756No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG62270.12930832195716357No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC59500.12355620935364112No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA57770.1199637346951235No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC55670.11560292730617146No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA54170.11248806488549143No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC53250.110577615934141No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA51260.10644523178937218No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT48190.10007014670171371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66100.077.4002461
GTATCAA130200.071.021751
ATCAACG161000.057.8646133
CAACGCA165500.057.42235
TCAACGC164200.057.131544
AACGCAG168600.056.067586
TATCAAC169450.054.3417472
ACGCAGA183300.051.4135137
CGCAGAG189200.049.8488128
GTACTGG46350.046.136391
GCAGAGT206050.045.253429
GGTTCAC44450.043.245826
TACTGGT48600.039.9974372
GACCGTT13550.038.255457
TTCACTA54750.034.978588
TCACTAT56300.034.658939
GTTCACT55700.034.640157
CTGGTTC55700.034.5112534
ACGGTAC12550.034.42013
TGGTTCA56750.034.1264465
Produced by FastQC (version 0.11.5)