FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004918919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885716
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85840.4552117073832963No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC65390.3467648362743913No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA61170.3243860687399375No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC58470.3100678999382728No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC53710.2848254986434861No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT51290.2719921769768088No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT49510.2625527916186743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46450.24632553364345428No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA45340.24043917535832546No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT39530.2096285973073358No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC37510.19891648583349772No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC36200.1919695224519493No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT35560.18857558614340653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35500.18825740461448065No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC32090.1701740877205263No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC31450.16678015141198357No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC31150.16518924376735417No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT30200.160151369559361No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA29200.15484834407726294No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG28990.15373470872602238No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA27670.14673471508965297No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG27660.14668168483483196No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA27280.1446665351516347No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT24320.1289695797246245No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG23650.1254165526516188No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG23570.12499231061305095No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC23430.12424988704555723No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT22840.12112110201111938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22520.11942413385684801No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC21590.11449232015849684No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG21480.11390898735546605No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT20640.10945444595050369No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC20210.10717414499320152No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC20150.10685596346427564No Hit
ATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATC20000.10606050964196093No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA19750.10473475327143643No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA19220.10192414976592445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)