FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991245
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84990.8574065947369218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55290.5577833936110649No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42680.4305696371734536No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.17937038774470487No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC16270.16413701960665628No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT16140.16282553758152626No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG12850.1296349540224667No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA12100.12206871156979354No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT11790.11894133135602197No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA11470.11571306790954809No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC10520.10612916080282876No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10360.10451502907959183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.075.644031
GTATCAA63100.062.8066441
ATCAACG82000.048.677973
TATCAAC84450.047.4770852
CAACGCA85150.046.8701025
TCAACGC85350.046.829964
AACGCAG88250.045.2910776
ATAGACC2350.040.501953
CGCAGAG102250.039.031688
ACGCAGA102500.038.936487
GCAGAGT104800.038.1387189
TATAGAC2700.035.2516942
GTACTGG6500.033.923581
GCTACCG2300.033.6181347
AGACCCG3000.031.7217255
CATGGGT9350.031.170414
GGCGTCG1551.9766958E-730.6984448
CGTAACC400.00536743329.7451234-35
CAGAGTA107800.029.5184210-11
GGTTCAC7400.028.13166
Produced by FastQC (version 0.11.5)