FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4348794
Sequences flagged as poor quality0
Sequence length125
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149880.34464727462372324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86810.1996185609159689No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58530.13458903778840753No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT55950.12865635852146595No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT47130.10837487358564236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43930.10101651170416441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA364900.047.060421
TATCAAC429000.040.001052
GGTATCA224750.037.660671
CAACGCA465000.037.2367825
ATCAACG464550.037.144813
AACGCAG478350.036.2228476
TCAACGC480300.036.1620564
ACGCAGA559250.030.9722867
CGCAGAG573000.030.2290578
GTCCTAG3350.030.184711
CATGGGT131550.030.0686344
ATGGGAG48900.029.5583575
ATGGGTG47200.029.4887755
GCAGAGT611100.028.393059
GCGCAGA48550.027.5661531
GTATACG3200.026.0232141
GTACATG695250.025.8288461
TACATGG695150.025.7812212
ACATGGG682800.025.6987153
GAGTACT108350.025.63736212-13
Produced by FastQC (version 0.11.5)