FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5866910
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA580500.9894475967758155No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC568690.9693177498887829No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC409330.6976926525206625No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG387100.6598021786596351No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA387070.6597510444169077No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC360140.6138495391952493No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC338890.5776294505966514No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA331090.564334547487519No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT304660.5192852796446511No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC284120.48427536812393573No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA279760.4768438581808822No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC261650.44597582032108896No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC251670.42896516224042974No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA245260.4180394790443351No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC231840.395165427797597No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA207140.3530649012853444No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA200980.342565336778645No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC183350.3125154468024906No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG178910.30494757887883056No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA168090.2865051620018033No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC164570.28050541085511793No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT157170.26789229764901795No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC152950.2606994141720258No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC148760.2535576649377611No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT145360.24776245076198544No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA141870.24181383385802746No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA139000.2369219913037698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127050.2165535179506759No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA126760.2160592202709774No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA124910.2129059419694524No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA123510.21051967730883892No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT122100.20811636790064955No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC121610.20728117526943485No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG120250.20496308959912457No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT118870.20261091443366266No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC118150.20138369260820432No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA117770.20073599220032354No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA112850.19234997639302462No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG108900.18561730110057936No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT108530.18498664544027435No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT108040.18415145280905962No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG103740.17682221135146098No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG103600.17658358488539963No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT103240.17596997397267045No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA102710.17506660235115248No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA102280.1743336782053926No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG101430.1728848746614487No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT99560.16969750686477209No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT98980.16870891150537504No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT98130.16726010796143115No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT96610.16466930632990792No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA95560.16287960783444777No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA92240.1572207516392786No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC91940.15670940921200427No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG90570.1543742787941182No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA87030.14834043815228118No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA85810.14626097894803225No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA85220.14525533884105943No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT84370.1438065352971155No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG83430.1422043290249893No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC83350.14206797104438282No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC82470.14056803325771147No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA76680.13069912441131704No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA75620.12889238116828108No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT75430.12856853096434068No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT75200.12817650177009704No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG73090.12458006003160096No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT72940.12432438881796379No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG71190.12134155799219692No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA71150.12127337900189367No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG69750.11888711434128016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69540.11852917464218814No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT69490.11844395090430909No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT68470.11670538665157638No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC67830.11561452280672449No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG66240.11290440794217058No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG65860.11225670753428978No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC65250.11121697793216531No Hit
GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA64910.11063745651458774No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT64240.10949545842700843No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT63730.10862617630064207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63000.10738190972760789No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA62960.10731373073730466No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA62570.10664898558184802No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT61580.10496155557184275No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT61530.1048763318339637No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT60710.10347866253274723No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC59480.10138215858092249No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG59320.10110944261970953No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG58730.10010380251273669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA171300.082.366221
GGTATCA93550.076.074551
TTCTCTG67550.061.5938727
TCTCTGC72050.057.5471048
AACGCAG267500.053.173866
ATCAACG271150.052.067643
ACGCAGA273050.051.723977
CGCAGAG274350.051.5313538
CAACGCA275850.050.975825
TATCAAC279300.050.8845022
GCAGAGT281350.050.3004239
CTCTGCA83000.050.2152339
TCAACGC281700.049.9889374
TATTCTC84900.049.866015
TCTCACA17800.048.8834232
ACTGTGC83150.045.7960938
ATTCTCT93750.045.45546
CTGTGCA78700.043.995219
CACTGTG78650.043.8401267
ATTTAGG164150.040.016861
Produced by FastQC (version 0.11.5)