FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6191431
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA236450.062.5121151
GGTATCA122200.046.8441931
CAACGCA324800.045.037935
TATCAAC329400.044.7637182
TCAACGC329500.044.4391674
AACGCAG332500.044.0272336
ATCAACG332750.043.9171453
ACGCAGA337250.043.3069537
CGCAGAG354150.041.1382688
GCAGAGT374700.038.8703049
AGAGTAC360050.030.73208210-11
CAGAGTA371300.027.29416310-11
AGTACGG406250.026.96499312-13
GAGTACG411000.024.4177712-13
TACGGGT76400.023.55074314-15
GTACGGG416900.023.34837714-15
TACGGGC110950.023.15949414-15
TACGGGG126450.022.1081114-15
TACGGGA165800.021.5965614-15
ACGGGAT36550.017.74202316-17
Produced by FastQC (version 0.11.5)