FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424702
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC27730.19463719430449317No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT24670.17315901851755666No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC21360.14992608980685082No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA20370.1429772682287243No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG20370.1429772682287243No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT19620.13771300945741638No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT19220.13490540477938545No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA19040.13364198267427152No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC18900.1326593210369607No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT17670.12402593665201565No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA17180.12058662092142777No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA17170.12051643080447702No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC17170.12051643080447702No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA16250.1140589400450059No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA15490.10872449115674716No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14910.10465346437360235No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC14740.1034602323854392No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC14600.10247757074812837No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT14380.10093338817521139No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14370.1008631980582606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63650.050.6380651
GGTATCA38550.050.2885861
ATCAACG82750.038.5215233
AGTATCG1104.7190042E-737.845315
CAACGCA84450.037.6052325
GTACTGG11750.037.4515721
TCAACGC86550.036.7615174
AACGCAG87550.036.4095546
ATATACG851.7155643E-434.983066
TATCAAC94250.034.072492
GACCGTT3150.033.9847347
ACGCAGA98900.031.9315627
CGCAGAG98750.031.8005058
GTACTAG3050.031.1958181
GCAGAGT100650.031.1432939
CTTAGTG2001.873559E-929.7356013
TTCACTA12100.029.0003918
GGTTCAC11550.028.3196186
ATGGGCC4900.027.9150545
GTTCACT12450.027.706437
Produced by FastQC (version 0.11.5)