FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004918983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004918983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022969
Sequences flagged as poor quality0
Sequence length125
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171230.4256309208447791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76690.19063035285631086No Hit
CTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72670.1806377329778082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49530.12311802551796944No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC49260.12244687940672673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152650.075.091011
CTATCAA82100.073.0194241
CTACGGG21350.069.86951
TATCAAC302450.058.3322872
CGTATCA10600.058.1956751
ATCAACG312400.056.2223783
TCAACGC315100.055.8289154
AACGCAG317250.055.573616
CAACGCA319800.055.0960925
GGTATCA51450.053.409021
ACGCAGA331900.053.0530137
CGCAGAG338050.052.15428
GCAGAGT348850.050.3788159
AGAGTAC346350.035.57962410-11
AGTACGG253250.032.37319612-13
CAGTACG12750.031.6684041
CAGAGTA350150.031.26976210-11
CTTATCA24400.031.2574061
GAGTACG251050.028.98243112-13
AGTACTT142150.028.7980912-13
Produced by FastQC (version 0.11.5)