FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958631
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC218140.7373004609226362No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA149520.5053688682366946No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC122730.41482023273601876No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115540.39051845262217555No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC112990.38189960153868463No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA112760.38112221497036974No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC102290.3457342264040362No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC101570.3433006684510505No Hit
ATCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT93810.317072321624427No Hit
ACCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA72880.24633014390777358No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA68650.23203299093398264No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC67820.2292276394048464No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC67200.2271320756119976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63050.21310531796631618No Hit
ACCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC59320.20049813579320974No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC56790.19194688354174616No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG54420.18393642194650162No Hit
ATATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG52050.17592596035125704No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG49480.1672395104357387No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA49030.16571853671512263No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA48950.1654481413870131No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG48920.16534674313897205No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT47820.16162880737746613No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA47710.16125701380131555No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC44680.15101579074916743No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA44030.1488188287082776No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG43450.14685846257948354No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT43370.14658806725137405No Hit
AACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT42910.14503329411474428No Hit
CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT42830.14476289878663476No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG42130.14239693966567646No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA41190.1392197945603896No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC41150.13908459689633482No Hit
ACTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT40480.13682003602341758No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT40250.13604264945510272No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG39370.133068300845898No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG39110.13218951602954204No Hit
AAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT39000.13181772245339146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38930.1315811265412956No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA38840.13127693179717242No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA38610.13049954522885754No Hit
ACTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38360.1296545598285153No Hit
TATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGC37330.1261732199791052No Hit
ACCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG36870.12461844684247544No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA36110.12204969122543502No Hit
ATACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT35690.12063011575286003No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT35270.11921054028028505No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG35010.1183317554639291No Hit
AATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG34840.11775716539169637No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA34560.11681078174331304No Hit
ACCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG34460.11647278758317614No Hit
ACATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG33610.11359983722201247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33580.11349843897397141No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT33340.11268725298964286No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA31660.1070089510993429No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT31640.10694135226731552No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT31170.10535277971467209No Hit
ATATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG30030.10149964628911141No Hit
AGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.10075605913681022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGT8750.099.967451
ACTATCA25300.097.2940141
AAGTACG13500.092.206451
ACCTAGA5750.085.813761
ACCCTAA11950.078.828541
AGTATCA19850.072.878811
ATATCAA84400.070.953021
ACTGTAC5800.070.572741
CTATCAA35350.070.426422
ACTCAGA9250.070.316681
ACTTTAT11050.065.459021
ACTATTA5600.065.082981
ATCTTAG21650.062.4167631
ATATACG7350.061.7930681
ACTTTCC7750.060.0507551
ACATAAA10750.055.2890971
ACCCTAC4700.053.685471
AACGGGA6850.053.206521
CCTAGAC2800.052.0663832
ATACTAG10500.049.1293071
Produced by FastQC (version 0.11.5)