FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3646105
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119270.3271161965988363No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA108210.2967824568957833No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC96230.2639254766387693No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG87970.24127116470864116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80910.22190803610976645No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA80860.22177090347096423No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG78150.21433831444788343No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG72330.19837607529130402No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT71950.19733386723640706No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC70520.19341187376666333No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA68080.18671980099311458No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT65330.179177505858992No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG65180.17876610794258532No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT63370.17380190641794463No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA63230.1734179350292984No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC61620.16900226405986662No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT57940.15890930184402258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54340.14903575185026213No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT52760.14470236046411172No Hit
CCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGG52760.14470236046411172No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG51870.14226139949343203No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA51190.1403963956057217No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA50310.1379828611628025No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCG49520.13581616546972727No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT48810.13386888199873564No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA47100.12917894575169941No Hit
CTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCG46380.12720423575294731No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC46120.12649114603117573No Hit
ACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTCCAAG45920.1259426154759668No Hit
CTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCG45150.12383077283841251No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC44610.12234974033934842No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC44500.12204804853398353No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCC44340.12160922408981638No Hit
GTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTA43720.11990877936866876No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACG42550.1166998756206966No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA41990.11516399006611165No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAACCGCCTG41810.11467031256642361No Hit
CTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTC41800.11464288603866318No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT41780.11458803298314227No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC41200.11299729437303643No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC40490.11105001090204478No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC40140.11009008243042918No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGT39330.10786853368183308No Hit
CGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGC38270.10496132173922583No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCG38170.10468705646162137No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC37300.1023009485464626No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGG37280.1022460954909417No Hit
CCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGT37210.10205410979661858No Hit
CCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTG36800.1009296221584403No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG36750.1007924895196381No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA36550.10024395896442917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81550.081.8363651
GTATCAA173250.065.324121
TATCAAC237000.048.3284342
ATCAACG244000.046.8841933
TCAACGC251750.045.416014
CAACGCA253750.045.034615
AACGCAG256700.044.517076
ACGCAGA268550.042.441967
CGCAGAG269800.042.289428
GCAGAGT279100.040.922919
CTCTGCA14650.032.890659
CAGAGTA279300.032.33150510-11
AGAGTAC277350.032.16202510-11
GAGTACT97200.029.47476812-13
ACTGTGC23550.028.5438548
TACGGAA4450.028.0788732
GGCGTCG4450.028.0727128
GTACGGA12400.027.3664111
CTGTGCA22850.027.0752339
TCTCTGC17050.026.8652828
Produced by FastQC (version 0.11.5)