FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389027
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.080.188081
GTATCAA52950.071.055731
TATCAAC77850.049.1577222
AACGCAG78900.048.4123576
CAACGCA81000.047.423655
ATCAACG80250.047.1497543
ACGCAGA81900.046.3755157
TCAACGC85200.044.410424
GCAGAGT86000.043.5806439
CGCAGAG87000.043.493158
TACGGGT23000.029.3994184
CAGAGTA80500.027.9706659
ACTGTGC12650.027.2963818
GAGTACG97550.026.9759671
AGTACGG97000.026.6974322
CACTGTG12600.022.8364287
GTGTAGG12750.022.174411
GTACGGG108350.021.311543
TACGGGC32300.020.934574
TAGGATC5900.020.726964
Produced by FastQC (version 0.11.5)