FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004919077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3434382
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA642841.871777804565712No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC580491.6902313138142464No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA492811.4349306512787454No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC425341.2384760926419949No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC399061.1619557754495569No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC320500.933210108834719No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC308950.8995796041325631No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG303240.8829536143620599No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC301350.8774504408653435No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA294410.8572430207239613No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC276960.8064332971696218No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT240270.6996018497651105No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA234310.6822479269923962No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG203540.5926539330802455No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC200400.5835110945724733No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT198360.5775711612744302No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC197750.5757950047490349No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC193350.5629833839101184No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA190950.5559952270888912No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA166780.48561866443511525No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA161050.46893444002443524No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG154260.4491637796843799No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG152980.4454367627130587No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT152370.4436606061876635No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA151080.4399044718962538No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA145250.42292907428468934No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA143230.41704737562682315No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA140020.4077007158784317No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA139950.4074968946378126No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT139590.4064486711146285No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC135110.39340411171500433No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA127340.3707799540062812No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC119500.3479519750569389No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115540.336421516301914No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT113300.3298992366021019No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT112080.3263469235513114No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG111060.32337695690228985No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT109370.31845612980734234No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA109150.3178155487653965No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG108020.31452529159540205No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC105780.30800301189558993No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA105380.30683831909205206No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT102660.2989184080279946No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT98780.28762088783367723No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT94690.2757119039175025No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG94170.2741978032729033No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA90190.2626091098777014No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG87270.254106852411875No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC82120.23911143256632486No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT81810.23820879564358305No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA79380.23113328686209045No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG77630.2260377558466123No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG77590.2259212865662585No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA77580.22589216924617006No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT76940.22402866076050945No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC76620.22309690651767916No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG75890.22097134215122255No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC73710.21462376637194114No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT70530.20536445858381508No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT69400.2020742014138206No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT68860.20050186612904447No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT68670.19994863704736396No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT68470.19936629064559505No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA68100.19828894980232253No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA68070.19820159784205718No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC67330.19604691615551212No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA67200.19566839099436228No Hit
TTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAG66630.19400870374932086No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA66570.19383399982879015No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA66390.19330988806719812No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT65920.19194137402304112No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT65860.19176667010251044No Hit
AATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT65380.190369038738265No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG64430.18760289332986255No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT64410.18754465868968567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64140.18675849104729758No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA63800.18576850216429042No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG62990.1834099992371262No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA62890.18311882603624174No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC62760.18274030087509194No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC62570.18218707179341145No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA62260.1812844348706696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61770.1798576861863357No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG58290.1697248587955562No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT57930.16867663527237214No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA57260.1667257748264462No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG56900.16567755130326212No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA55210.16075672420831463No Hit
TTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGT55000.16014526048645725No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG54790.15953379676459986No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC53710.1563891261950476No Hit
GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA53370.15539913731204041No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT52500.15286593046434555No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT52010.15143918178001167No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA51650.15039095825682758No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG51380.14960479061443951No Hit
CTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTA50960.14838186317072474No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA49860.14517895796099559No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG48290.14060753870710946No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT47630.13868579558127198No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT47150.13728816421702653No Hit
TGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTA46950.1367058178152576No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC45900.13364849920597066No Hit
CTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGG45750.13321173940464398No Hit
ATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCA43710.12727180610660083No Hit
GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC42780.1245638953383753No Hit
CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT42540.12386507965625256No Hit
GGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATA42200.12287509077324536No Hit
TATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTT41270.12016718000501983No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC41110.11970130288360468No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT40960.11926454308227798No Hit
GGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACC40280.1172845653162636No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT40260.11722633067608669No Hit
TAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGT40160.11693515747520224No Hit
ATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTT39510.1150425316694532No Hit
ATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAA39130.11393607350609221No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT38270.11143198397848579No Hit
GTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATAC38060.11082052025662842No Hit
TAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCT37870.11026729117494792No Hit
AAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGC37760.109947000653975No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG37580.10942288889238298No Hit
TATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTG37290.10857848660981802No Hit
ATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGAT36970.10764673236698771No Hit
GCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTT36810.10718085524557258No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA36670.10677321276433432No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT36610.10659850884380363No Hit
TTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTA36570.10648203956344984No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG36130.10520087747955817No Hit
AGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCAT35430.10316266507336691No Hit
GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT34380.10010534646408001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)