FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7082245
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT591360.834989470146825No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG345610.487994978993243No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA333320.4706417244814321No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT259450.3663386397957145No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT249990.35298129336107403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198490.2802642382464882No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT162450.22937641948280524No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG157400.2222459121366177No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT153620.21690862148937237No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG147640.20846497120616414No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT146280.206544676158478No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG141190.19935768954618205No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA139400.19683024238783042No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG116820.1649476966696295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115160.1626038071261302No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT114250.16131890382216374No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC105610.14911938234274585No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC103420.14602714252331003No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT98620.1392496305903001No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA98460.13902371352586645No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT98420.13896723425975804No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA98160.13860011903005331No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC96730.13658098526667745No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT96020.13557847829325306No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT94170.13296631223573882No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG89320.12611820122009335No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT89070.12576520580691575No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC86830.12260236690484444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86750.12248940837262762No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT84700.11959484598457128No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT83170.11743451405592435No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT78140.11033224634279103No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT78130.11031812652626392No Hit
CAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATA77890.10997925092961341No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC76090.1074376839547347No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC75670.10684465166059633No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC75180.10615278065076823No Hit
ATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACC73510.10379477129074184No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT73190.10334293716187451No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA71950.10159207991251361No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC71840.10143676193071548No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC71590.10108376651753787No Hit
TCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTT71440.1008719692696313No Hit
GCTAAATAGGTTGTTGTTGATTTGGTTAAAAAATAGTAGAGGGATGATGC70910.10012361899369479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG6450.064.548841
ATACGAC8400.051.6892053
GGTATCA157150.050.337011
GTATCAA323700.049.3897361
GCTCTAG3900.047.276711
GACACTC9350.044.529297
ATCAACG398100.040.2197343
CAACGCA403400.039.7945185
TCAACGC404550.039.6961024
AACGCAG419950.038.212086
TATCAAC422350.038.163122
GGGGTTA239500.036.233156
CTAGTGG1355.885522E-835.245721
GTACACT10750.034.856876
ACGCAGA468700.034.237847
CGCAGAG472050.033.982268
CTACATG7050.033.7459031
ATAGGGG244850.033.522413
AGGGGTT244400.033.4137845
GATCTAC8650.033.004661
Produced by FastQC (version 0.11.5)