FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004919097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383262
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66240.4788680669316442No Hit
GTACAAAGGGCAGGGACGTAATCAACGCATGCTGATGACACGCGCTTACT50040.36175359404075297No Hit
GCTCTATCCCCAGCACGACAGAGTTTAACAAGATTACCCAGACCTTTCGG40040.2894607095402028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35710.25815789055146454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32890.23777129712230943No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACAGAGCCAACGAGTTCTTCACC28810.20827580024608497No Hit
GGGCAGGGACGTAATCAACGCATGCTGATGACACGCGCTTACTAGGCATT27630.19974523987502008No Hit
ACACTGACAGAGCCAACGAGTTCTTCACCTTGGCCGAAAGGTCTGGGTAA26870.19425098065297824No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCATGCTGATGACACGCGCTTA24950.18037074682887264No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCATGCTGATGACACGCGCT24760.17899718202336218No Hit
GTACAATTGCAATACACCAGCTAGTAGCCCCAATTTCAATGAGGCTACCA23740.17162330780430604No Hit
GCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA23250.16808095646377907No Hit
GAGTACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCATGCTGA22380.16179147551223125No Hit
CTGTACAATTGCAATACACCAGCTAGTAGCCCCAATTTCAATGAGGCTAC21920.15846600282520593No Hit
ACCTTAACCTGCTAAATAGCCAGGCTAGCTTTGGCTGGTCGCCGGCTTCT21630.15636950917468997No Hit
GTCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCT20770.15015232110764265No Hit
GTATCAACGCAGAGTACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCA19800.1431399113110893No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA19770.14292303265758766No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC19470.14075424612257115No Hit
AATAAATACACCCCTTCGTAAGTTGGGGTTTTTAGCATGTATTAGCTCTA19300.1395252670860618No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGAT19280.1393806813170607No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC18900.13663355170603977No Hit
AGCTTTGGCTGGTCGCCGGCTTCTTAGAGGGACTATCGGCTCAAGCCGAT18420.13316349325001337No Hit
TACTAGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTATCCCCAG17920.12954884902498587No Hit
CTACACTGACAGAGCCAACGAGTTCTTCACCTTGGCCGAAAGGTCTGGGT17520.12665713364496387No Hit
TGGTAATACCGCTTAGTACGAGAGGAACCGCGGTTTCAGATAATTGGTTT17430.1260064976844589No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA16720.12087370288491985No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16680.12058453134691766No Hit
GTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTA16560.11971701673291105No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA16480.11913867365690664No Hit
GACGTAATCAACGCATGCTGATGACACGCGCTTACTAGGCATTCCTCGTT16300.11783740173589674No Hit
GGATAGAGCATTGCAATTATTGCTCTTCAACGAGGAATGCCTAGTAAGCG16160.11682530135288904No Hit
ATCCCCAGCACGACAGAGTTTAACAAGATTACCCAGACCTTTCGGCCAAG15050.10880079117332798No Hit
GGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGACA14540.1051138540637999No Hit
ACCCAGACCTTTCGGCCAAGGTGAAGAACTCGTTGGCTCTGTCAGTGTAG14470.10460780387229605No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT14380.10395716791179112No Hit
AGATTACCCAGACCTTTCGGCCAAGGTGAAGAACTCGTTGGCTCTGTCAG14100.10193296714577571No Hit
TCTTAGAGGGACTATCGGCTCAAGCCGATGGAAGTTTGAGGCAATAACAG14000.10121003830077022No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC13990.10113774541626966No Hit
GTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGC13840.1000533521487614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)