FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004919098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383262
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACGTAATCAACGCATGCTGATGACACGCGCTTACT43790.3165705412279091No Hit
GCTCTATCCCCAGCACGACAGAGTTTAACAAGATTACCCAGACCTTTCGG33370.24124135557833584No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACAGAGCCAACGAGTTCTTCACC26130.18890130719993753No Hit
ACACTGACAGAGCCAACGAGTTCTTCACCTTGGCCGAAAGGTCTGGGTAA25540.18463602701440507No Hit
GGGCAGGGACGTAATCAACGCATGCTGATGACACGCGCTTACTAGGCATT24580.17769591010235228No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCATGCTGATGACACGCGCTTA22800.16482777666125434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22470.16244211147273618No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCATGCTGATGACACGCGCT21890.15824912417170428No Hit
GTACAATTGCAATACACCAGCTAGTAGCCCCAATTTCAATGAGGCTACCA21790.1575261953266988No Hit
GAGTACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCATGCTGA21220.1534055009101674No Hit
ACCTTAACCTGCTAAATAGCCAGGCTAGCTTTGGCTGGTCGCCGGCTTCT21090.15246569341166027No Hit
GCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA19320.1396698528550629No Hit
CTGTACAATTGCAATACACCAGCTAGTAGCCCCAATTTCAATGAGGCTAC19080.13793482362704967No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGAT18810.13598291574553484No Hit
GTCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCT18700.1351876940160288No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC18500.13374183632601777No Hit
AATAAATACACCCCTTCGTAAGTTGGGGTTTTTAGCATGTATTAGCTCTA18280.13215139286700567No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC18090.1307778280614952No Hit
GTATCAACGCAGAGTACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCA17780.12853674864197814No Hit
AGCTTTGGCTGGTCGCCGGCTTCTTAGAGGGACTATCGGCTCAAGCCGAT17520.12665713364496387No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA17090.1235485396114402No Hit
TGGTAATACCGCTTAGTACGAGAGGAACCGCGGTTTCAGATAATTGGTTT16850.121813510383427No Hit
CTACACTGACAGAGCCAACGAGTTCTTCACCTTGGCCGAAAGGTCTGGGT16180.11696988712189013No Hit
GGATAGAGCATTGCAATTATTGCTCTTCAACGAGGAATGCCTAGTAAGCG15500.11205397097585273No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA15460.11176479943785053No Hit
TACTAGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTATCCCCAG15090.10908996271133019No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA15000.10843932675082522No Hit
GTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTA13870.10027023080226305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)