FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4248422
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC74890.17627721539903524No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194450.076.383181
GGTATCA82600.059.722171
TATCAAC283000.052.3989642
AACGCAG290700.050.868946
ATCAACG294250.050.1731533
ACGCAGA296100.049.7993857
TCAACGC296400.049.7289054
CAACGCA299100.049.200425
CGCAGAG301700.048.796148
GCAGAGT317400.046.32359
AGAGTAC306700.036.25014510-11
CAGAGTA312050.031.26206210-11
AGTACGG361450.030.11595712-13
GAGTACG363300.026.22853512-13
TACGGGC100100.026.00421114-15
TACGGGT85200.025.38426214-15
TACGGGA129350.024.99958814-15
GTACGGG385900.023.5970814-15
TACGGGG113350.021.78343214-15
ACGGGTA20750.019.78452516-17
Produced by FastQC (version 0.11.5)