FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15010983
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT1319340.8789164573699138No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT1079430.719093479754124No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA629030.4190465074805561No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT511290.34061060491508116No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG500710.33356243225377047No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT470140.3131973435717035No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT450460.30008694300699695No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA313590.2089070382665812No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT313270.20869386102162663No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG311640.20760798943013928No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA292000.19452423602105204No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG272470.18151376228991797No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT263170.17531829860842557No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT244380.162800797256249No Hit
TTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTA243040.16190811754300166No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT238030.15857056130168157No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG230600.15362085214539248No Hit
GGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCG225080.14994354466992602No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT217830.14511374771392385No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA213470.14220920775141774No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT211510.14090349712607098No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT210260.14007077351296712No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA209240.13939127104467441No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA202930.13518768224572633No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG202770.13508109362324908No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGA202550.13493453426734278No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC183870.12249031259311932No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA183630.12233042965940338No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC178530.1189329173179398No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA178320.11879301975093837No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA176710.11772047173726065No Hit
GTGTATGGTTATCATTTGTTTTGAGATTAGTTTGATTAGTCATTGTTGGG173130.1153355513093313No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC171740.11440956265155987No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT168930.11253759996930249No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAAT164900.10985289904065576No Hit
GCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATA164700.10971966326255916No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCT161660.10769447943549068No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT159160.10602903220928303No Hit
GTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGT159030.10594242895352023No Hit
GGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTT157280.10477661589517488No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT156790.1044501882388382No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC156150.10402383374892904No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTA153320.10213854748886199No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGC234350.093.119842
GTACATG236950.092.976671
CATGCTA247900.087.910014
ACATGCT261350.083.550863
TAAGACT288350.075.477549
CCCTGGG27750.063.565922
TGCTAAG348800.062.5217366
GGTATCA71050.057.681261
CGGGACA15700.057.3208734
GGGGTTA328850.055.9810566
CCTGGGG31600.055.135243
AACCGTG31850.054.25028
TAACCGT31900.054.166257
TTGTACG44800.054.156619
ATGCTAA407100.053.8687675
ACCGTGC32150.053.7439779
CAACGCA190900.052.9127045
CCGGATA17150.052.0547034
AACGCAG196850.051.3499376
CGCAGAG198150.050.9030238
Produced by FastQC (version 0.11.5)