FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13971271
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368140.2634978592856727No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249240.17839464999283172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173440.12414045937552853No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT145480.10412796373357872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG183150.059.471426
CAACGCA183000.059.3628125
CGCAGAG196250.055.4647258
ACGCAGA199750.054.492887
TCAACGC206900.053.2430844
GTATCAA217700.052.9898951
ATCAACG215500.051.1190383
GCAGAGT265900.041.071649
TATCAAC277850.040.244142
GTCTCGC10150.038.076311
GGTATCA119850.034.4976921
GAGTACG113950.033.0202521
GTACGGG146200.031.9709591
AGTACGG122250.030.9208972
ATAGGGG90250.029.917313
TAGGGGT92100.029.5504744
TACGGGG65950.028.6040862
CCGTGCG2553.266905E-928.231549
CGTCGAT1306.7602907E-427.6886279
GGGTTAG91850.027.1972587
Produced by FastQC (version 0.11.5)