FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13971271
Sequences flagged as poor quality0
Sequence length150
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170030.12169973655224353No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT143310.10257477648239735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG136050.054.0332836
CAACGCA137750.053.8893285
TCAACGC153300.048.2341544
CGCAGAG159800.045.77518
ATCAACG161400.045.5904353
ACGCAGA166050.044.3118367
GTATCAA177850.043.705481
GCAGAGT198600.036.2155889
GAGTACG112650.034.7572631
TATCAAC231550.033.2118642
AGTACGG120550.032.4339182
GTACGGG139600.030.5312141
TACGGGA70300.027.9623762
TAGGGGT99800.026.9094414
ATAGGGG98850.026.0755043
GGTATCA111400.025.6795541
GGGTTAG101550.024.6012557
TACGGGG63850.024.359012
GGGGTTA103600.024.0464236
GTCTCGC10700.023.6298921
Produced by FastQC (version 0.11.5)