FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4123691
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA165150.063.8802381
GGTATCA78450.055.1414031
CAACGCA227800.045.5570375
ATCAACG230100.045.30693
AACGCAG229900.045.2449846
ACGCAGA229850.045.0978477
CGCAGAG231300.044.892318
TATCAAC233850.044.8099172
TCAACGC233900.044.5708354
GCAGAGT252450.041.0585759
AGAGTAC243050.032.53473310-11
CAGAGTA255900.028.33173610-11
AGTACGG273900.028.19513912-13
TACGGGT64350.026.21159414-15
GAGTACG280300.025.30140912-13
TACGGGC64650.025.0316414-15
TACGGGG77300.024.89910514-15
GTACGGG279650.024.44523214-15
TACGGGA102300.022.56550614-15
ACGGGTA20400.020.41559816-17
Produced by FastQC (version 0.11.5)