FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004919225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44494020
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3152740.7085761187683199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1465330.3293318967357861No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140980.25643446018139066No Hit
GCCCAGGAGAGGGCAGCTCTGAACTGCTGGCTGGGACCAGCTCAGTAGGA1011600.22735639530885277No Hit
CAGTAAAAGTGGTGGGCATCCCTGGGTGCCAGACCTGCCGCTACCTCCTG810340.18212335050867512No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA775940.17439197447207513No Hit
CTTTTACTGTGTCCTCTTCCTCAGGACACGTAAGGTTTTTGTCCACCATA749300.16840465302977792No Hit
GTCTATCTCAGTGCCAGATATGGTGGACAAAAACCTTACGTGTCCTGAGG713050.16025749078190732No Hit
CGGGTACACAGGGCCACGCAGTGACCACCGCGGGACCAGGGCTGGTGAGC705170.1584864662711978No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC683960.15371953354630577No Hit
GCTCTGAACTGCTGGCTGGGACCAGCTCAGTAGGAACAGATGAAAGGAAG654810.14716809135250086No Hit
GAGGTGGAGGGAGGGATGGCAAGCAGAGGAGGGGAGGCAGCTGCCCAGGA642750.14445761475362306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620200.13938951796218907No Hit
ACACAGGGCCACGCAGTGACCACCGCGGGACCAGGGCTGGTGAGCAGCCC565720.12714517591352725No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA557120.12521233190437728No Hit
GGGCCACGCAGTGACCACCGCGGGACCAGGGCTGGTGAGCAGCCCAGTAC541080.12160735307800913No Hit
GCCTGAGGATGAGGAGACACCAGAGCAGGAGATGGAGGAGACCCCTTGCA526030.11822487606199664No Hit
GTCCAGGGGCCCAGCCTCTGGGCCCAGTAACTCAGCTACTCTTTGTGGCT510600.11475699431069614No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC507140.11397936172096834No Hit
CTTTATTATTCTGCAGGTACATGTCCAGGGGCCCAGCCTCTGGGCCCAGT498450.11202629027451329No Hit
GTGCTACAGGGGCAACCTGGTTTCCATCCACAACTTCAATATTAATTATC498400.11201505280934382No Hit
GAGATAGACTCAACAGCCCCATCTTTCTTGGAGGCATCTTCACTTCCAGA485360.10908432189314428No Hit
GCTCAGTAGGAACAGATGAAAGGAAGTCTTCTGAGGCAGTGGGCTCGACG484720.10894048233897498No Hit
CTGTAGCACCTCCGGCAAGTAAACCAAGCTTGACTAAACGTCTGAAGACT478920.10763693637931568No Hit
GAGCTGGAGGAAGAGGAGGAGTGGGGCTCTGGAAGTGAAGATGCCTCCAA471700.10601424640884327No Hit
GACAGAACACTGGATTCGATAATTAATATTGAAGTTGTGGATGGAAACCA457820.10289472607779653No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)