FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004919250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715147
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52530.30627112428264164Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47410.27641945559185305Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT28010.1633096171931619No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22540.13141730708796387Clontech Universal Primer Mix Long (96% over 26bp)
TTCTCATTCTTCCTTCCTGTCTTGCTACCCTCACAGCCTCATCCAAAAGA21800.12710280809749835No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT20690.12063105961180004No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC20340.11859041819739066No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19740.11509217577268886Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGT18990.11071937274181165No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)