FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11097290
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT623940.5622453770244807No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA272650.24569061455544552No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG255730.23044364885481053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232470.20948357662095882No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT219160.19748965738482097No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC215060.1937950616772203No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT210810.18996529783397567No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT208570.1879467870083597No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT199550.17981867645163818No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA198950.17927800390906248No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC179190.16147185484023577No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC166520.15005465298284537No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA157840.14223292353358344No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT157020.1414940043920633No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT156610.1411245448213032No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT145210.13085176651236471No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG144760.13044626210543295No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC141070.12712112596859232No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG138880.12514767118819098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137710.12409335973016837No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT134860.12152516515293373No Hit
GCTAAATAGGTTGTTGTTGATTTGGTTAAAAAATAGTAGAGGGATGATGC131690.11866861188632541No Hit
GTATTATTCCTTCTAGGCATAGTAGGGAGGATATGAGGTGTGAGCGATAT129980.11712769513998463No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT129900.1170556054676412No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG129490.11668614589688113No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT128600.11588414829206048No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG125910.11346013305951273No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT125780.11334298734195465No Hit
TAATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAA122310.11021609780405847No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA122090.11001785120511405No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT121480.10946816745349541No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG120510.10859408017633133No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC119280.10748570146405112No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC115740.10429573346285445No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT111900.1008354291903699No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135350.078.622761
GCTCTAG4550.057.5224651
ATACGAC6800.056.8604663
GTATCAA430400.053.886151
GTTCTAT9750.053.0775451
GTACTAA6150.050.2949521
ATCAACG449800.050.0820163
TCAACGC459250.048.999674
CAACGCA460150.048.9296845
ATATACG6400.048.3303071
AACGCAG483400.046.6501586
GTCCCGT1950.045.75651
TATCAAC518950.043.7514882
TACATGC45700.043.6038442
ACGCAGA528750.042.63787
GTACTAG6900.042.241871
CGCAGAG534600.042.2157338
GCATAGA6250.040.9246181
TACTGAT28650.040.901362
GCAGAGT557150.040.4643949
Produced by FastQC (version 0.11.5)