FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11097290
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT536380.4833432306445988No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG343960.30994954624056864No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA290170.2614782527986562No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT284710.2565581326612173No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT268880.24229338874626147No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC206370.18596432101891544No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT204480.18426120250980194No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC201560.18162992946926682No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT198910.17924195907289076No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC192350.1733306059407297No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA185060.16676143454843478No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG156500.141025421521831No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT152170.13712356800624298No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT148840.13412283539494776No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG145930.13150057356345557No Hit
CTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTAGAAGGAAT145400.13102297948418037No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC132690.11956973279061826No Hit
TAATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAA131850.11881279123101227No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA129810.11697450458625484No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG128450.11574898015641656No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG127910.11526237486809844No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG127790.1151542403595833No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT125570.11315375195205316No Hit
GCTAAATAGGTTGTTGTTGATTTGGTTAAAAAATAGTAGAGGGATGATGC124610.11228867588393202No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT124480.11217153016637395No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA123730.1114956894881543No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT123080.11090996090036398No Hit
GTATTATTCCTTCTAGGCATAGTAGGGAGGATATGAGGTGTGAGCGATAT118280.10658458055975828No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT117910.10625116582516994No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA117500.10588170625440987No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC117330.10572851570068008No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT114730.10338560134951866No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT114490.10316933233248837No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC112650.10151126986858955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89350.066.5714951
GCTCTAG4750.065.116741
ATATACG8450.049.9786531
ATACGAC9250.046.940783
GTATCAA313300.046.6474231
ATCAACG303600.046.0399933
CAACGCA304900.045.77985
TCAACGC306750.045.525564
AACGCAG327500.042.5428246
TACATGC44500.040.633362
TCCAGTA10750.039.8376052
ACGCAGA366000.038.1259737
CGCAGAG369850.037.712178
TACTAGA20750.037.552261
TATCAAC382700.037.1767922
GCAGAGT384550.036.378139
GGGGTTA233900.036.101356
GTACACT12750.035.446126
CTACTGG28100.034.4867749
TAGATGG32750.033.4037677
Produced by FastQC (version 0.11.5)