FastQCFastQC Report
Wed 31 Jul 2024
EGAF00004919459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50305330
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC2141230.42564674558342025TruSeq Adapter, Index 11 (100% over 50bp)
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG616200.12249199041135402No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG549430.10921904299206467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)