FastQCFastQC Report
Mon 5 Aug 2024
EGAF00004919603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42973823
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT899810.20938560667502168TruSeq Adapter, Index 18 (97% over 40bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG525170.12220695375414936No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG498510.11600317709690386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)