FastQCFastQC Report
Wed 31 Jul 2024
EGAF00004919621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57997245
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC4515310.7785387047264055TruSeq Adapter, Index 8 (100% over 50bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG651420.11231912826204073No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG607230.10469980082674618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)