FastQCFastQC Report
Wed 31 Jul 2024
EGAF00004919679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49777308
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT3595960.72240949631105TruSeq Adapter, Index 16 (97% over 40bp)
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG703320.14129329774121172No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA675650.13573453992329196No Hit
GGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCT559460.11239257856210302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)