FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4626527
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA167000.070.1949841
GGTATCA72300.061.069161
CAACGCA229800.050.0986675
ATCAACG230200.049.9906433
TCAACGC231150.049.8103874
TATCAAC234850.049.4838372
AACGCAG234150.049.0477186
ACGCAGA237000.048.4147
CGCAGAG243750.047.096188
GCAGAGT260500.043.8881079
AGAGTAC248250.034.99598310-11
CAGAGTA253400.031.25650810-11
AGTACGG279250.030.85289812-13
GAGTACG280000.027.9555812-13
TACGGGT52850.027.01178714-15
GTACGGG293250.026.1255214-15
TACGGGG101450.025.97399714-15
TACGGGA103350.025.43893214-15
TACGGGC74900.024.26143614-15
ACGGGAT25300.021.75086216-17
Produced by FastQC (version 0.11.5)