FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777923
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186060.38941607053943733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95760.20042181508576007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87260.1826316581493674No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC73330.15347673037007922No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA229550.071.436051
GGTATCA98900.068.449531
TATCAAC352000.048.8441352
ATCAACG359150.048.086863
TCAACGC362400.047.557224
AACGCAG369800.046.6357546
CAACGCA371100.046.4413265
ACGCAGA390150.044.1880267
CGCAGAG396450.043.4708448
GCAGAGT417100.041.3329129
AGAGTAC412550.032.843310-11
AAGTACG12500.030.9062541
CAGAGTA419350.029.10799210-11
AGTACGG344950.028.6327712-13
GAGTACG344450.025.70683112-13
TACGGGC88100.024.46937814-15
TACGGGT83750.024.28466614-15
GAGTACT139700.023.88591212-13
AGTACTT155200.023.52982112-13
GTACGGG361300.023.48804314-15
Produced by FastQC (version 0.11.5)