FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777923
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC68860.14412120078117627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA196800.073.869711
GGTATCA84100.061.417451
TATCAAC296650.048.823692
ATCAACG295750.048.690073
TCAACGC297000.048.4034844
AACGCAG306050.047.2161336
CAACGCA306250.046.9525575
ACGCAGA311650.046.3911747
CGCAGAG315950.045.7409748
GCAGAGT332650.043.269049
AGAGTAC325500.034.18877810-11
CAGAGTA330900.029.49664110-11
AGTACGG387550.028.5896912-13
TACGGGT92550.025.93165214-15
GAGTACG389400.024.92576612-13
TACGGGC99600.024.72316414-15
TACGGGA147700.022.83313814-15
GTACGGG408150.022.77729814-15
TACGGGG117800.021.35787814-15
ACGGGTA22950.020.6080216-17
Produced by FastQC (version 0.11.5)