FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004919958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976738
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC113200.38028204027361495No Hit
GTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACG93730.31487487309934564No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT81790.2747638522436304No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC63750.2141606013024996No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT63120.21204419065433372No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA54900.18443007076874082No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT52340.17583005289682868No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG52140.17515817650058554No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG50910.17102613666369026No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC49890.16759956704285026No Hit
TTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACG47720.16030970814361223No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC47710.16027611432380007No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA45530.1529526616047499No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA43130.1448901448498323No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC42020.14116123085068286No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG41390.139044820202517No Hit
GCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGA40890.13736512921190916No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC39520.13276277589764365No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT39200.13168777366365464No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT37680.12658151305220683No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC36530.12271822377380877No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA33950.11405101826227233No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC33140.11132991885748764No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT33050.1110275744791782No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC31320.10521584365167508No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG30700.10313302682332136No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA30630.10289787008463627No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC30600.1027970886251998No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG30540.10259552570632685No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG30410.10215880604876881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)