FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004919965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037922
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC343381.1303121014956934No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC339421.1172768754431484No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG334991.102694539227801No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC271140.8925179777492641No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA248230.8171045866220398No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA233630.7690454198626562No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT224330.738432388981679No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA214750.7068976754505218No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA201520.6633481702295188No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA178360.5871118481646336No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC166450.5479074182944789No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA150490.4953715072342213No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA149310.49148727320846286No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC138400.45557456708895094No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG134310.4421114169488222No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA127770.4205835436196189No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG125090.41176172396789645No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG119340.3928343124017009No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC118190.38904883008846175No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT112290.3696276599596698No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA111310.36640177068403995No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA103040.33917921526622474No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG101040.3325957677649393No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA99520.3275923476639624No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT92580.304747784834502No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT90370.2974730753455816No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG89200.29362175855732964No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT89140.2934242551322911No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC88000.2896716900565584No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA81780.26919716832756074No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC79520.2617578726511082No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT78710.2590915764130876No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT76720.25254104614930867No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG73440.24174419224720056No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC73020.24036166827193065No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA72230.23776120650892285No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA71500.23535824817095372No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA70350.2315727658577146No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC64340.2117895061163519No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC64010.21070323727863982No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA61550.20260559685205873No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA60270.1983921904512361No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC59370.19542963907565764No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC58470.1924670877000792No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG58030.1910187292497964No Hit
ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC57890.19055788792470643No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT57330.18871452262434651No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA57320.1886816053868401No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC55900.18400735766092743No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC53810.1771276550220842No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT53080.174724696684115No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC52500.17281549690874223No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA51850.17067587647082447No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC51620.16991878000817665No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT51010.16791082852028458No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA49650.1634340842194105No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC49080.16155780168154416No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA45690.15039885816686538No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG44820.14753505850380622No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT44660.14700838270370337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42560.1400957628273537No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA42200.13891074227712233No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT42050.1384169837145259No Hit
TCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCA41600.1369357080267367No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC39540.13015475710041272No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC39190.12900265378768777No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG38850.12788346771246925No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA38320.1261388541246286No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT38200.12574384727455148No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT37310.12281421313647947No Hit
AATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGG37180.12238628904889592No Hit
GATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATG37060.12199128219881879No Hit
ACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCT36890.12143168916120954No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC35580.11711953104786758No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA35050.11537491746002695No Hit
GTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATAC33970.11181985580933282No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG33560.11047024907156931No Hit
TAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGT33430.11004232498398576No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG31940.10513765659552812No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT31600.1040184705203096No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG31020.10210927074493684No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA30730.10115467085725045No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG30450.10023298820707048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)