FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7134743
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA300400.038.7586941
ATACGAC7800.033.560873
CAACGCA351350.032.4780465
ATCAACG353050.032.3043563
TCAACGC356200.032.1522944
AACGCAG357000.032.0924076
TATCAAC365100.031.7596112
CATGGGT88950.028.8943524
CGCAGAG399100.028.4899948
ACGCAGA402950.028.3113027
GTACATG614750.027.7561241
ACATGGG569600.027.4701423
ATAGGGG40050.027.33323
TACATGG615500.027.306472
CATGGGG143850.026.9657754
GCAGAGT425200.026.7831579
GGGGTTA40950.026.2929236
GGTATCA195450.026.2695941
TAGGGGT43800.024.8571974
GGGTTAG45300.024.421777
Produced by FastQC (version 0.11.5)