FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4976462
Sequences flagged as poor quality0
Sequence length125
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354640.7126347995825146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91640.184146889898888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88150.1771338754319836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA259750.080.311091
CGTACGG17900.059.4755672
GCTATCA46100.058.9493331
GCGTACG18550.058.2977031
GGTATCA77850.057.5077251
TATCAAC366800.056.7654952
ATCAACG385850.056.7487953
TCAACGC392950.056.0080454
AACGCAG400700.055.0086826
CAACGCA401600.054.8714565
ACGCAGA429450.051.2999767
CGCAGAG439850.050.163448
GCAGAGT462400.047.535359
CTATCAA56700.046.545312
AGAGTAC454400.032.41051510-11
CAGAGTA461050.031.64529610-11
GCGTAAG4850.030.0379091
GAGTACT209900.027.45415712-13
AGTACGG325200.027.39446412-13
GAGTACG328050.026.7807212-13
Produced by FastQC (version 0.11.5)