FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004919990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004919990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4976462
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA197050.073.6224751
GGTATCA79950.060.212021
TATCAAC276800.052.2209972
TCAACGC284050.050.6995744
ATCAACG283850.050.67243
AACGCAG288900.049.848946
CAACGCA289950.049.668425
ACGCAGA293900.048.855217
CGCAGAG306550.046.8003438
GCAGAGT319450.044.8691449
AGAGTAC308700.034.58727610-11
CAGAGTA314950.030.27373310-11
AGTACGG354600.029.5136912-13
GAGTACG355500.026.30093612-13
TACGGGT76650.025.96452314-15
TACGGGC90800.024.47378514-15
GTACGGG375350.023.99067314-15
TACGGGA143300.023.33385814-15
TACGGGG115650.022.84198214-15
ACGGGTA18050.020.7661216-17
Produced by FastQC (version 0.11.5)