FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004920005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186817
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT6043032.34716326672626No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG158218.468715373868545No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC63173.3813839211634917No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT15310.8195185662974996No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT13870.7424377867110594No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT13640.730126273304892No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT5120.27406499408512075RNA PCR Primer, Index 26 (100% over 22bp)
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT4300.23017177237617562No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCG4050.2167896925868631No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCG3930.21036629428799306No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.20929572790484804No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCG3870.20715459513855808No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC3700.19805478088182554No Hit
NTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT2710.14506174491614787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.13970891300042287No Hit
ACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA2280.12204456767853032No Hit
CTTATACACATCTCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC2200.1177623021459503RNA PCR Primer, Index 26 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC2180.11669173576280532RNA PCR Primer, Index 26 (95% over 22bp)
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT2170.11615645257123282No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT1960.10491550554821027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)