FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004920006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186817
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG142157.6090505682031075No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT44822.3991392646279515No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG18570.9940208867501351No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT3120.16700835577062045No Hit
GTGATTCCATTCTATTCTATTCAATGGTGATTCCATTCGAGTACATTAGA3100.16593778938747544No Hit
GTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATAT2600.13917362980885037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)