FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004920036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194653
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA50210.42028940621251526No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC46920.39275002866941283No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT37720.31574021912639066No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC36900.3088763013192952No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT35250.2950647593903837No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT32850.2749752438574214No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT31740.2656838429234263No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT31440.263172653481806No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG30710.25706209250719664No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG30660.25664356093359325No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA29960.25078411890314595No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG29820.24961223049705647No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC29250.24484097055797793No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA28310.23697257697423438No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG27800.23270355492347986No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA27360.2290204770757701No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT27120.2270115255224739No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC25670.21487410988797587No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC25610.2143718719996518No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG25570.21403704674076907No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC25490.21336739622300366No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24440.20457823317733265No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC23530.19696095853775114No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA23270.19478459435501355No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT23210.19428235646668948No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC23170.19394753120780678No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC22340.18699990708599065No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC22010.18423759870020834No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG21470.17971745770529185No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT21460.17963375139057114No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACC20690.17318836515707908No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG19800.16573850314693891No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG19730.16515255894389416No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA19530.16347843264948064No Hit
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA18880.1580375221926367No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17820.149164652832245No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA17350.14523045604037324No Hit
GTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCC17010.14238444133987024No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT16890.14137996556322213No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC16740.140124370842412No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC16410.13736206245662966No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTG16240.13593905510637816No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC16180.13543681721805412No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG16100.1347671667002887No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15940.1334278656647579No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC15640.1309166762231376No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG15530.12999590676121014No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT15320.12823807415207597No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA14960.1252246468221316No Hit
ATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCT14810.12396905210132146No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT14460.1210393310860978No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG14440.12087191845665646No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCA14240.11919779216224292No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA13950.11677030903534333No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC13480.11283611224347154No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA13470.11275240592875087No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCACCCGG13270.11107827963433735No Hit
GGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGT13240.11082716069017531No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG13220.11065974806073395No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA13190.11040862911657194No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA12630.10572107549221404No Hit
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCG12630.10572107549221404No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCAC12610.1055536628627727No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA12590.10538625023333134No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA12400.1037958302536385No Hit
ACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTC12210.10220541027394565No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG12150.10170317238562158No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG12140.10161946607090092No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAA12060.10094981555313551No Hit
CGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGC12050.10086610923841484No Hit
GACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTG12020.1006149902942528No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC12010.10053128397953214No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA11990.10036387135009077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)