FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10350389
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1493991.443414348967947No Hit
ACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA851290.8224715032449506No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG618300.5973688525136591No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT538370.5201447018078257No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT485260.46883262068701No Hit
CCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAG424020.4096657623206239No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT391230.3779857935774201No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA305550.29520629611118965No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT282820.27324576883052415No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT211830.20465897465302996No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT203760.19686216624322045No Hit
AGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCAT198420.19170293986052117No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA196010.1893745249574678No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT189740.18331678162047824No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT189220.1828143850438858No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA185080.17881453537639985No Hit
ATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAAT183120.17692088674155146No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT182960.17676630317952302No Hit
CTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGAT178470.17242830197009987No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC177660.1716457226873309No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT173360.16749128945781652No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT171580.1657715473302501No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC163370.15783947830366568No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG161500.15603278292245826No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG159990.15457390055581485No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA158830.15345316973110865No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA152960.1477818852991902No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT149750.14468055258599458No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA147190.14220721559353955No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG146690.14172414196220065No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT145270.14035221284919822No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA142770.13793684469250383No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC136510.13188876282814105No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC134210.12966662412398222No Hit
ATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACC129550.12516437787990384No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT129280.12490351811898084No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG127640.12331903660818933No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC124480.12026601125812758No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC122870.11871051416521639No Hit
TCTGAGATGTTAGTGTTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC122820.1186622068020825No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC122520.11837236262327917No Hit
TCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTT121810.11768639806677798No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT121290.11718400149018553No Hit
TAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCT116890.1129329535344034No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC116740.11278803144500171No Hit
GGTTAGTTGTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAG115390.11148373264038675No Hit
ATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTA114970.1110779507900621No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC113920.11006349616425044No Hit
GTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATG112640.10882682766802292No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT112430.10862393674286058No Hit
AAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCA109170.10547429666653108No Hit
CAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATA108120.10445984204071944No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG107640.10399609135463411No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT104810.10126189460125605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG85900.076.077531
TACATGC87400.072.985992
CGGGGAC10800.068.6619344
CATGCTA101750.062.3367354
CGGGACA15800.060.603424
CAACGCA133050.059.0909275
ATCAACG136350.057.817523
AACGCAG137650.057.325446
TCAACGC137150.057.323064
ACATGCT110800.057.2448733
CCGCAGT20100.056.8253561
GGTATCA41900.056.5901641
TAAGACT112400.056.4951449
GGGGTTA421500.056.196776
CGCAGAG140500.056.0595868
GGGTTAG424100.056.005047
ACGCAGA142900.055.11867
AGGGGTT418550.055.0791245
CGCAGTA21200.054.3377152
ATAGGGG429250.054.3251763
Produced by FastQC (version 0.11.5)