FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4633339
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220380.47563970605215805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111620.2409061801866861No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103110.2225392961749615No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA96650.2085968671836876No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC81920.17680553915869313No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC76520.1651508771536035No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT68890.14868327139456017No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT61850.13348904537311No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61480.13269048519868717No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAAAAAAAAAAAAAAAAAAAAAAA60220.12997106406416625No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA56180.12125165026776587No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC52630.11358978913479027No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC51210.11052504468160003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA294500.069.452651
ACAGAGA28200.061.7779247
GGTATCA188000.050.7692681
TATCAAC424700.048.0770652
CAACGCA425400.047.434325
ATCAACG442650.045.670583
TCAACGC449500.044.9745984
AACGCAG450750.044.9103746
ACGCAGA456950.044.269517
CGCAGAG457300.044.2356268
CAGAGAA39800.043.77238
GCAGAGT481000.042.1907359
AATACAG43950.041.2801784
TGAAATA48400.039.651661
AGAGAAA44850.038.6831329
CTATTGA43350.037.6967439
ATTTAGG44850.036.9329871
AAAGGTC25550.036.3467757
GAAATAC55100.035.149132
TTTAGGG47900.034.5705452
Produced by FastQC (version 0.11.5)