FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4131388
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA96640.23391654330215417No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC77350.18722521341495885No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC75350.18238422534993082No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG72290.17497751361043792No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA66710.16147115690900976No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCA66620.16125331244608349No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG65700.1590264579361706No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA59540.1441162146958843No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT59370.14370473071035691No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC59110.14307540226190327No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT58310.14113900703589205No Hit
GTATCAACGCAGAGTACGGGCCCCCGTCAATTCATTTGAGTTTTAACCTT57220.1385006685404518No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT56310.13629801897086402No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAG53360.12915756157494768No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAG52330.12666445272145826No Hit
CCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCA51370.1243407784502448No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGA50680.12267063756781015No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC48780.11807169890603351No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGT45770.11078601186816635No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG45060.10906746110508139No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44160.10688901647581879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.084.337421
GTATCAA158100.061.598161
ATCAACG212950.045.369563
TATCAAC219250.044.33672
CAACGCA221450.043.4937975
TCAACGC221550.043.4473154
AACGCAG223550.043.2704626
ACGCAGA226700.042.5380067
CGCAGAG228700.042.2419978
GCAGAGT233950.041.2940569
CAGAGTA238250.032.89324610-11
AGAGTAC240550.031.51705710-11
GTACTGG26700.027.6287561
ACTGTGC23350.026.2406718
CTTTCTA11150.026.1424229
ACGGGTT23500.025.8219185
GAGTACG304600.025.55273212-13
TCTAGAC1651.1119457E-525.2388923
ACGGGTA13000.025.12782116-17
CTGTGCA25050.024.9348169
Produced by FastQC (version 0.11.5)