FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004920097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272643
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44080.3463657915063376No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT35810.28138291728316583No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG34560.27156083834979644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.249637958170516No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT30130.23675139060993539No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT29840.23447266829739372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24860.19534150582685011No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA23410.18394789426414163No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA23060.1811977121627982No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC19880.1562103433563065No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT19200.15086713241655358No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT18660.14662399431733802No Hit
ATCTATCACCCTATAGAAGAGCTAATGTTAGTATAAGTAACATGAAAACA16980.13342312023088956No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG16500.12965144192047573No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC15700.12336531140311932No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA15570.12234381519404891No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC15110.11872929014656898No Hit
TTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTA14550.11432899878441952No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG14540.11425042215295256No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT14380.11299319604948128No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA14010.11008586068520394No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG13880.10906436447613353No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT13760.10812144489853005No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC13440.10560699269158749No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT13150.10332827037904582No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAAT13050.10254250406437626No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC12960.10183531438117367No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT12790.10049951164623544No Hit
ATATTATACTAGCATTTGCCATCTCACTTCTAGGAATACTAGTATATCGC12770.10034235838330152No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC12740.10010662848890066No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)