FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004920099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620024
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC79800.4925852950326662No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT59950.370056246080305No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT49690.3067238510046765No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG44160.2725885542436408No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42580.2628356123119164No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT39970.24672473988039684No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT37160.22937931783726662No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT34620.2137005377698108No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA30940.19098482491617408No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC29760.183700982207671No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC28340.1749356799652351No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG26090.16104699683461482No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG25850.159565537300682No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA25290.15610879838817204No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC24160.1491335930825716No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC23960.14789904347096092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.14117074808768265No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT22290.13759055421401165No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA21720.13407208782092117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21380.131973353481183No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG20480.12641788022893488No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG20080.1239487810057135No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC20040.12370187108339135No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA19960.12320805123874708No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG19610.12104758941842836No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT19150.11820812531172378No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG18150.11203537725367031No Hit
TGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGAC18060.1114798299284455No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT17870.11030700779741534No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG17800.1098749154333516No Hit
TTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCGTGTTTGC17500.10802309101593556No Hit
GTTCCGACCTGCACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGA17230.10635644904026112No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAA17180.10604781163735845No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT16830.10388734981703976No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGAT16770.10351698493355654No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG16510.10191207043846265No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT16270.10043061090452982No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)