FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434805
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336930.5236056104264232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150620.23407080711847525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134060.2083357615343433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA347700.071.312421
GGTATCA144100.064.2428741
ATCAACG475400.051.7648853
TATCAAC479300.051.492432
TCAACGC480150.051.239624
AACGCAG490350.050.2441146
CAACGCA490100.050.174335
ACGCAGA513450.048.0172357
CGCAGAG524700.046.987718
GCAGAGT559900.044.1291669
AGAGTAC549000.033.7350510-11
CAGAGTA557150.030.3892510-11
AGTACGG447050.028.37858212-13
GAGTACT209250.026.6166312-13
AGTACTT225200.025.8526412-13
GAGTACG449350.025.28505712-13
TACGGGT102100.025.22077814-15
TACGGGC107900.024.05797614-15
TACGGGA173550.023.23278814-15
GTACTTT240650.023.19234714-15
Produced by FastQC (version 0.11.5)