FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434805
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA247450.067.695951
GGTATCA110950.053.90271
ATCAACG349150.047.5481343
TCAACGC351500.047.127944
AACGCAG352350.047.070456
CAACGCA355950.046.482455
TATCAAC359600.046.4660382
ACGCAGA361600.045.886017
CGCAGAG370450.044.724548
GCAGAGT395750.041.853579
AGAGTAC387400.033.0001710-11
CAGAGTA393400.028.57365610-11
AGTACGG452900.027.96285412-13
GAGTACG454200.024.51776512-13
TACGGGC112500.024.2147514-15
TACGGGT104000.023.73459814-15
TACGGGA173850.023.0938414-15
GTACGGG470350.022.54744114-15
TTAGGGC45600.022.052773
TACGGGG133950.021.64706214-15
Produced by FastQC (version 0.11.5)