FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5130544
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACG215370.41978004671629365No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC168350.328132845171974No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA162790.31729578773712885No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC145240.28308888881958716No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG136490.26603416713705214No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC130560.254475938613917No Hit
TTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACG119750.23340604816955082No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC113430.22108766633713697No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT113080.22040547746983555No Hit
GCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGA112530.2193334663926476No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA108750.2119658266257925No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA107070.2086913200627458No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT103760.20223976248912395No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC97290.18962901399929522No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC97130.18931715623138598No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT96120.1873485540714591No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT94890.18495114748065702No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA92810.18089699649783728No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC87730.17099551236671978No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA84490.16468039256655825No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA79790.15551957063422514No Hit
CCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGAC76480.14906801306060333No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG73350.14296729547587936No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC72920.14212917772462336No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT69790.1360284601398994No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA69190.13485899351023986No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG65020.12673120043410602No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA62380.12158554726360402No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC62020.12088386728580829No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG60860.1186228984684665No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG60840.11858391624747786No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT59070.11513398968998219No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT58570.1141594341652659No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC56030.10920869209970717No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA55530.10823413657499088No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG53150.10359525227734134No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT52700.10271815230509669No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC52150.10164614122790878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA195950.074.260151
GGTATCA91800.065.516871
TATCAAC312300.047.1463282
ATCAACG318500.045.761613
TCAACGC322300.045.203614
AACGCAG323850.045.0056276
ACGCAGA329800.044.2297447
CGCAGAG331300.044.0833638
CAACGCA331500.043.841425
GCAGAGT336700.043.411699
TCTCTGC28650.041.321228
CTCTGCA30600.038.6880049
TATAAGA42550.037.8891072
ATAAGAG45500.034.1250843
AGAGTAC342350.033.47675310-11
AAGAGAC41600.033.4631775
TAAGAGA50600.033.0369874
GACAGTC18500.032.7999429
TCTATAC42800.032.663953
ACTGTGC71000.032.510088
Produced by FastQC (version 0.11.5)